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Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination

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posted on 2023-10-13, 14:01 authored by Dinesh Aggarwal, Diana Rajan, Katherine L. Bellis, Emma Betteridge, Joe Brennan, Catarina de Sousa, Julian Parkhill, Sharon J Peacock, Marcus C. de Goffau, Josef Wagner, Ewan M. Harrison

Supplementary materials for "Optimisation of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination", as described in Microbial Genomics, including:

Supplementary Figures

Supplementary Tables

Supplementary Methods and Results

CARRIAGE study authorship list

ENA accession numbers and sample metadata

STORM guidelines checklist

Taxonomy tables

Funding

Wellcome Trust 222903/Z/21/Z

Defining the biological basis of Staphylococcus aureus carriage

Wellcome Trust

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Wellcome Sanger Institute Quinquennial Review 2021-2026

Wellcome Trust

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History